| 2009 Top League Challenge Series | |
|---|---|
| Countries | Japan |
| Date | 11 – 25 January 2009 |
| Champions | Ricoh Black Rams (1st title) |
| Runners-up | Honda Heat |
| Promoted | Honda Heat Ricoh Black Rams |
| Matches played | 6 |
The 2009 Top League Challenge Series was the 2009 edition of the Top League Challenge Series, a second-tier rugby union competition in Japan, in which teams from regionalised leagues competed for promotion to the Top League for the 2009–10 season. The competition was contested from 11 to 25 January 2009.
Honda Heat and Ricoh Black Rams won promotion to the 2009–10 Top League, while Mazda Blue Zoomers and Toyota Industries Shuttles progressed to the promotion play-offs.