| 2016 Columbus Challenger | |
|---|---|
| Date | 19–25 September |
| Edition | 2nd |
| Draw | 32S / 16D |
| Prize money | $50,000 |
| Surface | Hard / Indoors |
| Location | Columbus, United States |
| Champions | |
| Singles | |
 Mikael Torpegaard | |
| Doubles | |
 Miķelis Lībietis /  Dennis Novikov | |
The 2016 Columbus Challenger 1 was a professional tennis tournament played on indoor hard courts. It was the second edition of the tournament which was part of the 2016 ATP Challenger Tour. It took place in Columbus, United States between 19 and 25 September 2016.