| 2016 Estoril Open | |
|---|---|
| Date | 25 April – 1 May |
| Edition | 2nd |
| Draw | 28S / 16D |
| Prize money | €463,520 |
| Surface | Clay |
| Location | Cascais, Portugal |
| Venue | Clube de Ténis do Estoril |
| Champions | |
| Singles | |
 Nicolás Almagro | |
| Doubles | |
 Eric Butorac / Scott Lipsky | |
The 2016 Estoril Open (also known as the Millennium Estoril Open for sponsorship purposes) was a tennis tournament played on outdoor clay courts. It was the second edition of the Estoril Open, and part of the ATP World Tour 250 series of the 2016 ATP World Tour. The event took place at the Clube de Ténis do Estoril in Cascais, Portugal, from 25 April until 1 May 2016. Unseeded Nicolás Almagro won the singles title.