3,3',5,5'-Tetramethylbenzidine

3,3′,5,5′-Tetramethylbenzidine
Names
	Preferred IUPAC name 3,3′,5,5′-Tetramethyl[1,1′-biphenyl]-4,4′-diamine
	Other names 3,3′,5,5′-Tetramethylbiphenyl-4,4'-diamine
Identifiers
CAS Number	54827-17-7 ;
3D model (JSmol)	Interactive image;
ChEMBL	ChEMBL1331128;
ChemSpider	37605 ;
ECHA InfoCard	100.053.949
EC Number	259-364-6;
PubChem CID	41206;
UNII	3B3T5CB8EO ;
CompTox Dashboard (EPA)	DTXSID5026120 ;
	InChI InChI=1S/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 Key: UAIUNKRWKOVEES-UHFFFAOYSA-N ; InChI=1/C16H20N2/c1-9-5-13(6-10(2)15(9)17)14-7-11(3)16(18)12(4)8-14/h5-8H,17-18H2,1-4H3 Key: UAIUNKRWKOVEES-UHFFFAOYAM;
	SMILES Cc1cc(cc(c1N)C)c2cc(c(c(c2)C)N)C;
Properties
Chemical formula	C16H20N2
Molar mass	240.3482 g/mol
Melting point	168 to 171 °C (334 to 340 °F; 441 to 444 K)
Hazards
	GHS labelling:
Pictograms
Signal word	Warning
Hazard statements	H302, H315, H319, H335
Precautionary statements	P261, P264, P270, P271, P280, P301+P312, P302+P352, P304+P340, P305+P351+P338, P312, P321, P330, P332+P313, P337+P313, P362, P403+P233, P405, P501
Safety data sheet (SDS)	External MSDS
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). verify (what is ?) Infobox references

3,3′,5,5′-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).^[1] TMB is a white solid that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights. Used to detect hematuria as it turns blue in contact with hemoglobin.

^ Sigma Aldrich Catalog Entry for 3,3′,5,5′-Tetramethylbenzidine

[1] Sigma Aldrich Catalog Entry for 3,3′,5,5′-Tetramethylbenzidine

[1]