The 38th General Assembly of Prince Edward Island was in session from March 29, 1916, to June 26, 1919. The Conservative Party led by John Alexander Mathieson formed the government. Aubin-Edmond Arsenault succeeded Mathieson as Premier and party leader in 1917.
John S. Martin was elected speaker. Albert P. Prowse became speaker in 1918.
There were five sessions of the 38th General Assembly:
| Session | Start | End |
|---|---|---|
| 1st | March 29, 1916 | May 4, 1916 |
| 2nd | August 4, 1916 | August 5, 1916 |
| 3rd | March 15, 1917 | April 26, 1917 |
| 4th | March 26, 1918 | April 26, 1918 |
| 5th | April 2, 1919 | May 4, 1919 |