6-phosphogluconolactonase

6-phosphogluconolactonase
Crystallized monomer of 6-phosphogluconolactonase from Trypanosoma brucei complexed with 6-phosphogluconic acid[1]
Identifiers
SymbolPGLS
NCBI gene25796
HGNC8903
OMIM604951
RefSeqNM_012088
UniProtO95336
Other data
EC number3.1.1.31
LocusChr. 19 p13.2
Search for
StructuresSwiss-model
DomainsInterPro

6-Phosphogluconolactonase (EC 3.1.1.31, 6PGL, PGLS, systematic name 6-phospho-D-glucono-1,5-lactone lactonohydrolase) is a cytosolic enzyme found in all organisms that catalyzes the hydrolysis of 6-phosphogluconolactone to 6-phosphogluconic acid in the oxidative phase of the pentose phosphate pathway:[2]

6-phospho-D-glucono-1,5-lactone + H2O = 6-phospho-D-gluconate

The tertiary structure of 6PGL employs an α/β hydrolase fold, with active site residues clustered on the loops of the α-helices. Based on the crystal structure of the enzyme, the mechanism is proposed to be dependent on proton transfer by a histidine residue in the active site.[1] 6PGL selectively catalyzes the hydrolysis of δ-6-phosphogluconolactone, and has no activity on the γ isomer.[3]

  1. ^ a b Delarue M, Duclert-Savatier N, Miclet E, Haouz A, Giganti D, Ouazzani J, Lopez P, Nilges M, Stoven V (February 2007). "Three dimensional structure and implications for the catalytic mechanism of 6-phosphogluconolactonase from Trypanosoma brucei". Journal of Molecular Biology. 366 (3): 868–81. doi:10.1016/j.jmb.2006.11.063. PMID 17196981.
  2. ^ Berg J, Tymoczko J, Stryer L (2012). Biochemistry (Seventh ed.). New York, NY: W.H. Freeman and Company. pp. 600–601. ISBN 9781429229364.
  3. ^ Miclet E, Stoven V, Michels PA, Opperdoes FR, Lallemand JY, Duffieux F (September 2001). "NMR spectroscopic analysis of the first two steps of the pentose-phosphate pathway elucidates the role of 6-phosphogluconolactonase". The Journal of Biological Chemistry. 276 (37): 34840–6. doi:10.1074/jbc.M105174200. PMID 11457850.