ACMI may refer to:
- A Certain Magical Index, a Japanese light novel series
- Air Combat Maneuvering Instrumentation, a set of systems that record an aircraft's in-flight data during weapon range operations
- American College of Medical Informatics, a college of elected fellows who have made significant and sustained contributions to the field of medical informatics
- ACMI (Australian Centre for the Moving Image), an Australian institution dedicated to the moving image in all its forms
- Art & Creative Materials Institute, Inc., an industry trade group composed of companies that manufacture art materials
- Aircraft, Crew, Maintenance and Insurance, a type of lease contract used in aviation, more commonly known as a Wet lease
- Archdiocesan Commission for the Pastoral Care of Migrants and Itinerant People, a non-profit social service organization in Singapore