ATFS may refer to:
- Artificial transcription factor, an example of a chimeral protein designed to target and modulate gene transcription.
- Association of Track and Field Statisticians, an international organisation run by volunteers whose goal is to collect and disseminate the statistics of Track and Field Athletics.
- After The Forking Show, the explicit podcast created by the team from Spoonman's Classic Rock Show.
- American Tree Farm System, a certification program for tree farms
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