Activity-based proteomics

Fluorophosphonate-rhodamine (FP-Rhodamine) activity-based probe for profiling of the serine hydrolase superfamily. In this probe the fluorophosphonate is the reactive group (RG) as it binds irreversibly to the active-site serine nucleophile of serine hydrolases and the tag is rhodamine, a fluorophore for in-gel visualization.

Activity-based proteomics, or activity-based protein profiling (ABPP) is a functional proteomic technology that uses chemical probes that react with mechanistically related classes of enzymes.[1]

  1. ^ Berger AB, Vitorino PM, Bogyo M (2004). "Activity-based protein profiling: applications to biomarker discovery, in vivo imaging and drug discovery". American Journal of Pharmacogenomics. 4 (6): 371–81. doi:10.2165/00129785-200404060-00004. PMID 15651898. S2CID 18637390.