Altamura Cathedral (Italian: Duomo di Altamura, Cattedrale di Santa Maria Assunta), dedicated to the Assumption of the Blessed Virgin Mary, is a Roman Catholic cathedral in the city of Altamura, in the Metropolitan City of Bari, Apulia, in southern Italy.
Since 1986 it has been the seat of the Bishop of Altamura-Gravina-Acquaviva delle Fonti, formed in that year. Previously it was the church of the territorial prelature of Altamura (from 1848, Altamura e Acquviva delle Fonti).