Bartolo is an Italian surname. Notable people with the surname include:
- Andrea di Bartolo (1360/70–1428), 14th century Siennese painter
- Andrea di Bartolo (c. 1421 – 1457), 15th century Florentine painter
- Andrea del Bartolo (1460–1524), 15th century Milanese painter
- Augusto Bartolo (1883–1937), Maltese politician, journalist, and judge
- Evarist Bartolo (born 1952), Maltese politician
- Ivan Bartolo, Maltese politician
- Ivan J Bartolo, Maltese politician
- Jaiden Bartolo (born 2006), Gibraltarian footballer
- Sal Bartolo (1917–2002), American boxer
- Yorman Polas Bartolo (born 1985), Cuban basketball player