Biosurfactant usually refers to surfactants of microbial origin.[1] Most of the biosurfactants produced by microbes are synthesized extracellularly and many microbes are known to produce biosurfactants in large relative quantities.[2] Some are of commercial interest.[3] As a secondary metabolite of microorganisms, biosurfactants can be processed by the cultivation of biosurfactant producing microorganisms in the stationary phase on many sorts of low-priced substrates like biochar, plant oils, carbohydrates, wastes, etc. High-level production of biosurfactants can be controlled by regulation of environmental factors and growth circumstances. [4]
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