The Bristol and Gloucestershire Railway was an early mineral railway, opened in two stages in 1832 and 1834, which connected collieries near Coalpit Heath with Bristol, at the river Avon. Horse traction was used. It was later taken over by the Bristol and Gloucester Railway, and much of the route became part of the main line between Birmingham and Bristol, though that was later by-passed and closed. Part of it now forms the Bristol and Bath Railway Path.
This railway should not be confused with the later Bristol and Gloucester Railway, which opened in 1844.