CCR4-Not

Structure of human CNOT1-CNOT2-CNOT3 module (PDB 4C0D).[1]

Carbon Catabolite Repression—Negative On TATA-less, or CCR4-Not, is a multiprotein complex that functions in gene expression.[2][3] The complex has multiple enzymatic activities as both a poly(A) 3′-5′ exonuclease and a ubiquitin ligase.[4][5][6][7] The exonuclease activity of CCR4-Not shortens the poly(A) tail found at 3' end of almost every eukaryotic mRNA.[8] The complex is present both in the nucleus where it regulates transcription and in the cytoplasm where it associates with translating ribosomes and RNA processing bodies.[2][3] In mammalian cell, it has a function in the regulation of the cell cycle, chromatin modification, activation and inhibition of transcription initiation, control of transcription elongation, RNA export, nuclear RNA surveillance, and DNA damage repair in nucleus. Ccr4–Not complex plays an important role in mRNA decay and protein quality control in the cytoplasm.[9]

  1. ^ Boland, Andreas; Chen, Ying; Raisch, Tobias; Jonas, Stefanie; Kuzuoğlu-Öztürk, Duygu; Wohlbold, Lara; Weichenrieder, Oliver; Izaurralde, Elisa (2013-10-13). "Structure and assembly of the NOT module of the human CCR4–NOT complex". Nature Structural & Molecular Biology. 20 (11). Springer Science and Business Media LLC: 1289–1297. doi:10.1038/nsmb.2681. ISSN 1545-9993. PMID 24121232. S2CID 24288483.
  2. ^ a b Collart, Martine A. (2016-01-29). "The Ccr4-Not complex is a key regulator of eukaryotic gene expression". Wiley Interdisciplinary Reviews: RNA. 7 (4). Wiley: 438–454. doi:10.1002/wrna.1332. ISSN 1757-7004. PMC 5066686. PMID 26821858.
  3. ^ a b Miller, Jason E.; Reese, Joseph C. (2012-03-15). "Ccr4-Not complex: the control freak of eukaryotic cells". Critical Reviews in Biochemistry and Molecular Biology. 47 (4). Informa UK Limited: 315–333. doi:10.3109/10409238.2012.667214. ISSN 1040-9238. PMC 3376659. PMID 22416820.
  4. ^ Aslam, Akhmed; Mittal, Saloni; Koch, Frederic; Andrau, Jean-Christophe; Winkler, G. Sebastiaan (2009). Wickens, Marvin (ed.). "The Ccr4–Not Deadenylase Subunits CNOT7 and CNOT8 Have Overlapping Roles and Modulate Cell Proliferation". Molecular Biology of the Cell. 20 (17). American Society for Cell Biology (ASCB): 3840–3850. doi:10.1091/mbc.e09-02-0146. ISSN 1059-1524. PMC 2735483. PMID 19605561.
  5. ^ Chen J, Chiang YC, Denis CL (Mar 2002). "CCR4, a 3′-5′ poly(A) RNA and ssDNA exonuclease, is the catalytic component of the cytoplasmic deadenylase". EMBO J. 21 (6): 1414–26. doi:10.1093/emboj/21.6.1414. PMC 125924. PMID 11889047.
  6. ^ "Entrez Gene: CNOT6 CCR4-NOT transcription complex, subunit 6".
  7. ^ Albert, Thomas K.; Hanzawa, Hiroyuki; Legtenberg, Yvonne I.A.; de Ruwe, Marjolein J.; van den Heuvel, Fiona A.J.; Collart, Martine A.; Boelens, Rolf; Timmers, H.Th.Marc (2002-02-01). "Identification of a ubiquitin-protein ligase subunit within the CCR4-NOT transcription repressor complex". The EMBO Journal. 21 (3). Wiley: 355–364. doi:10.1093/emboj/21.3.355. ISSN 0261-4189. PMC 125831. PMID 11823428.
  8. ^ Stowell, James A. W.; Webster, Michael W.; Kögel, Alexander; Wolf, Jana; Shelley, Kathryn L.; Passmore, Lori A. (2016-11-15). "Reconstitution of Targeted Deadenylation by the Ccr4-Not Complex and the YTH Domain Protein Mmi1". Cell Reports. 17 (8): 1978–1989. doi:10.1016/j.celrep.2016.10.066. ISSN 2211-1247. PMC 5120349. PMID 27851962.
  9. ^ Chalabi Hagkarim, Nafiseh; Grand, Roger J. (2020-10-29). "The Regulatory Properties of the Ccr4–Not Complex". Cells. 9 (11): 2379. doi:10.3390/cells9112379. ISSN 2073-4409. PMC 7692201. PMID 33138308.