COS cells

COS-7 cells, 40X magnification

COS are fibroblast-like cell lines derived from monkey kidney tissue. COS cells are obtained by immortalizing CV-1 cells[1] with a version of the SV40 virus that can produce large T antigen but has a defect in genomic replication.[2] The CV-1 cell line in turn was derived from the kidney of the African green monkey.[3]

The acronym "COS" is derived from the cells being CV-1 (simian) in Origin, and carrying the SV40 genetic material.[2] Three COS lines were created (COS-1, COS-3 and COS-7), of which two are commonly used (COS-1 and COS-7).

  1. ^ Jensen FC, Girardi AJ, Gilden RV, Koprowski H (July 1964). "Infection of human and simian tissue cultures with rous sarcoma virus". Proc. Natl. Acad. Sci. U.S.A. 52 (1): 53–9. Bibcode:1964PNAS...52...53J. doi:10.1073/pnas.52.1.53. PMC 300571. PMID 14192657.
  2. ^ a b Gluzman Y (January 1981). "SV40-transformed simian cells support the replication of early SV40 mutants". Cell. 23 (1): 175–82. doi:10.1016/0092-8674(81)90282-8. PMID 6260373. S2CID 36241020.
  3. ^ Fanelli, Alex (2016). "COS-7 cell line: Origin and Characteristics". Retrieved 28 November 2017.