| Part of a series on |
| CRISPR |
|---|
| Genome editing: CRISPR-Cas |
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variants: Anti-CRISPR - CIRTS - CRISPeYCRISPR-Cas10 - CRISPR-Cas13 - CRISPR-BEST CRISPR-Disp - CRISPR-Gold - CRISPRa - CRISPRi Easi-CRISPR - FACE |
| Enzyme |
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Cas9 - FokI - EcoRI - PstI - SmaI HaeIII - Cas12a (Cpf1) - xCas9 |
| Applications |
| CAMERA - ICE - Genética dirigida |
| other Genome editing method: |
| Prime editing - Pro-AG - RESCUE - TALEN - ZFN - LEAPER |
CRISPR interference (CRISPRi) is a genetic perturbation technique that allows for sequence-specific repression of gene expression in prokaryotic and eukaryotic cells.[1] It was first developed by Stanley Qi and colleagues in the laboratories of Wendell Lim, Adam Arkin, Jonathan Weissman, and Jennifer Doudna.[2] Sequence-specific activation of gene expression refers to CRISPR activation (CRISPRa).
Based on the bacterial genetic immune system - CRISPR (clustered regularly interspaced short palindromic repeats) pathway,[3] the technique provides a complementary approach to RNA interference. The difference between CRISPRi and RNAi, though, is that CRISPRi regulates gene expression primarily on the transcriptional level, while RNAi controls genes on the mRNA level.
- ^ Jensen TI, Mikkelsen NS, Gao Z, Foßelteder J, Pabst G, Axelgaard E, et al. (November 2021). "Targeted regulation of transcription in primary cells using CRISPRa and CRISPRi". Genome Research. 31 (11): 2120–2130. doi:10.1101/gr.275607.121. PMC 8559706. PMID 34407984.
- ^ Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA (February 2013). "Repurposing CRISPR as an RNA-guided platform for sequence-specific control of gene expression". Cell. 152 (5): 1173–1183. doi:10.1016/j.cell.2013.02.022. PMC 3664290. PMID 23452860.
- ^ Barrangou R, Fremaux C, Deveau H, Richards M, Boyaval P, Moineau S, et al. (March 2007). "CRISPR provides acquired resistance against viruses in prokaryotes". Science. 315 (5819): 1709–1712. Bibcode:2007Sci...315.1709B. doi:10.1126/science.1138140. hdl:20.500.11794/38902. PMID 17379808. S2CID 3888761.