The Calais Branch is a mothballed railroad line in Maine that was operated by the Maine Central Railroad Company (MEC).
The Calais Branch is 127 miles (204 km) long and connects Brewer to Calais. It was constructed in 1898 and carried freight and passengers over the years. Passenger service was discontinued in 1957 and freight service was discontinued over the majority of the western end of the line in 1984. The line also includes a spur to Eastport which joins the Calais Branch at Ayers Junction.