Clover yellow mosaic virus

Clover yellow mosaic virus
Virus classification Edit this classification
(unranked): Virus
Realm: Riboviria
Kingdom: Orthornavirae
Phylum: Kitrinoviricota
Class: Alsuviricetes
Order: Tymovirales
Family: Alphaflexiviridae
Genus: Potexvirus
Species:
Clover yellow mosaic virus
Synonyms
  • Pea mottle virus

Clover yellow mosaic virus (ClYMV) is a plant pathogenic virus[1] in the genus Potexvirus and the virus family Alphaflexiviridae. Its flexuous rod-shaped particles measure about 539 nm in length.

Like other members of the Potexvirus genus, ClYMV is a monopartite strand of positive-sense, single-stranded RNA surrounded by a capsid made for a single viral encoded protein. The genome has been completely sequenced [2] and is 7015 nucleotides long. No insect vector is known. This virus is transmitted by mechanical inoculation, sometime by seeds and by dodder (Cuscuta campestris).

Potexviruses make banned inclusions[3] made up of layers of parallel virus particles. These inclusions can be seen in the light microscope in leaf strips of infected plant tissue stained with Azure A or Orange-Green stains.[4][5] For many potexviruses these inclusions can be disrupted during the staining procedures. The banded inclusions of ClYMV (Fig.1) however, remain stable and therefore can be diagnostic.[6] Antiserum is available for this virus [7] and, as mentioned above, so is sequence data.


Figure 1. Banned inclusions body (I) of Clover yellow mosaic virus in Vicia faba cells, stained with Azure A.(N = nucleus)

  1. ^ "About Plant Viruses". Archived from the original on 2014-10-10. Retrieved 2014-10-08.
  2. ^ "Clover yellow mosaic virus, complete genome". 2018-08-13. Retrieved 14 January 2009.
  3. ^ "Potexvirus Inclusions". Archived from the original on 2014-10-13. Retrieved 2014-10-08.
  4. ^ "Materials and Methods for the Detection of Viral Inclusions". Archived from the original on 2014-10-13. Retrieved 2014-10-08.
  5. ^ Christie, R.G. and Edwardson, J.R. (1977). Fla Agric. Exp. Stn Monog. No. 9, 150 pp.
  6. ^ "How do you diagnose a virus infection in a plant?". Archived from the original on 2014-10-09. Retrieved 2014-10-08.
  7. ^ "DSMZ". Archived from the original on 2011-01-10. Retrieved 2009-01-15.