Clover yellow mosaic virus | |
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Virus classification | |
(unranked): | Virus |
Realm: | Riboviria |
Kingdom: | Orthornavirae |
Phylum: | Kitrinoviricota |
Class: | Alsuviricetes |
Order: | Tymovirales |
Family: | Alphaflexiviridae |
Genus: | Potexvirus |
Species: | Clover yellow mosaic virus
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Synonyms | |
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Clover yellow mosaic virus (ClYMV) is a plant pathogenic virus[1] in the genus Potexvirus and the virus family Alphaflexiviridae. Its flexuous rod-shaped particles measure about 539 nm in length.
Like other members of the Potexvirus genus, ClYMV is a monopartite strand of positive-sense, single-stranded RNA surrounded by a capsid made for a single viral encoded protein. The genome has been completely sequenced [2] and is 7015 nucleotides long. No insect vector is known. This virus is transmitted by mechanical inoculation, sometime by seeds and by dodder (Cuscuta campestris).
Potexviruses make banned inclusions[3] made up of layers of parallel virus particles. These inclusions can be seen in the light microscope in leaf strips of infected plant tissue stained with Azure A or Orange-Green stains.[4][5] For many potexviruses these inclusions can be disrupted during the staining procedures. The banded inclusions of ClYMV (Fig.1) however, remain stable and therefore can be diagnostic.[6] Antiserum is available for this virus [7] and, as mentioned above, so is sequence data.
Figure 1. Banned inclusions body (I) of Clover yellow mosaic virus in Vicia faba cells, stained with Azure A.(N = nucleus)