Columbina is a stock character and Harlequin's mistress
Columbina may also refer to:
- Columbina (bird), a genus of dove
- Columbina Rural Service Center (or Columbina Township), Zhombe, Kwekwe, Midlands, Zimbabwe; a township
- Flora (Francesco Melzi) also called La Columbina; a 1520 painting by Francesco Melzi