Cross-linking immunoprecipitation

Cross-linking and immunoprecipitation (CLIP, or CLIP-seq) is a method used in molecular biology that combines UV crosslinking with immunoprecipitation in order to identify RNA binding sites of proteins on a transcriptome-wide scale, thereby increasing our understanding of post-transcriptional regulatory networks.[1][2][3] CLIP can be used either with antibodies against endogenous proteins, or with common peptide tags (including FLAG, V5, HA, and others) or affinity purification, which enables the possibility of profiling model organisms or RBPs otherwise lacking suitable antibodies.[4]

  1. ^ Ule, Jernej; Jensen, Kirk B.; Ruggiu, Matteo; Mele, Aldo; Ule, Aljaz; Darnell, Robert B. (2003-11-14). "CLIP identifies Nova-regulated RNA networks in the brain". Science. 302 (5648): 1212–1215. Bibcode:2003Sci...302.1212U. doi:10.1126/science.1090095. ISSN 1095-9203. PMID 14615540. S2CID 23420615.
  2. ^ Cite error: The named reference :7 was invoked but never defined (see the help page).
  3. ^ Ule, Jernej; Hwang, Hun-Way; Darnell, Robert B. (2018-08-01). "The Future of Cross-Linking and Immunoprecipitation (CLIP)". Cold Spring Harbor Perspectives in Biology. 10 (8): a032243. doi:10.1101/cshperspect.a032243. ISSN 1943-0264. PMC 6071486. PMID 30068528.
  4. ^ Lee, Flora C. Y.; Ule, Jernej (2018-02-01). "Advances in CLIP Technologies for Studies of Protein-RNA Interactions". Molecular Cell. 69 (3): 354–369. doi:10.1016/j.molcel.2018.01.005. ISSN 1097-4164. PMID 29395060.