Cruickshank ( KRUUK-shank) or Cruickshanks is a Scottish surname. Notable people with the surname include:
- Adrian Cruickshank (1936–2010), Australian politician
- Alexander M. Cruickshank (1919–2017), American chemist
- Andrew Cruickshank (1907–1988), Scottish actor
- Art Cruickshank (1918–1983), American special effects artist
- Bobby Cruickshank (1894–1975), Scottish professional golfer
- Clare Cruickshank (died 2013), British cystic fibrosis sufferer remembered in Little Yellow Duck Project
- Dan Cruickshank (born 1949), British architectural historian and television presenter
- Daron Cruickshank (born 1985), Professional MMA fighter
- Frank Cruickshank (1931–2015), Scottish professional footballer
- George Cruickshank (disambiguation), several people
- Gord Cruickshank (1965–2021), Canadian ice hockey player
- Grahame Cruickshanks (1913–1941), South African cricketer
- Harry Cruickshank Harley (1926–2014), Canadian politician
- Helen Cruickshank (1886–1975), Scottish poet and suffragette
- Helen G. Cruickshank (1902–1994), American nature writer and photographer of birds
- Jamie Cruickshank (born 1986), Canadian bobsledder
- Jessi Cruickshank (born 1983), Canadian television personality
- Jim Cruickshank (1941–2010), Scottish international football goalkeeper
- Jim Cruickshank (bishop) (1936–2015), Canadian bishop
- Joanna Cruickshank (1875–1958), British founder of the RAF Nursing Service in 1918
- John Cruickshank (disambiguation), several people
- Jorge Cruickshank García (1915–1989), Mexican politician
- Mandy-Rae Cruickshank (born 1974), Canadian free-diver
- Margaret Cruickshank (1873–1918), New Zealand medical practitioner
- Martin Melvin Cruickshank (1888–1964), Scottish surgeon, Chief Medical Officer for Delhi Province, Commander of the Indian Empire in 1942.
- Nancy Cruickshank, British entrepreneur in beauty, fashion, and technology
- Robert Cruickshank (disambiguation), several people
- Roger Cruickshank (born 1982), Scottish downhill skier and RAF pilot
- Sheena Cruickshank, British immunologist
- Stephen Cruickshank (born 1983), Trinidadian soccer player
- William Cruickshank (chemist) (died 1810), Scottish chemist and military surgeon