Dihydrolipoyl transacetylase

DLAT
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 1FYC, 1Y8N, 1Y8O, 1Y8P, 2DNE, 2PNR, 2Q8I, 3B8K, 3CRK, 3CRL
Identifiers
Aliases	DLAT, DLTA, PDC-E2, PDCE2, dihydrolipoamide S-acetyltransferase, Dihydrolipoyl transacetylase, E2
External IDs	OMIM: 608770; MGI: 2385311; HomoloGene: 6814; GeneCards: DLAT; OMA:DLAT - orthologs
Gene location (Human) Chr. Chromosome 11 (human)[1] Band 11q23.1 Start 112,024,814 bp[1] End 112,064,390 bp[1]
Gene location (Mouse) Chr. Chromosome 9 (mouse)[2] Band 9|9 A5.3 Start 50,545,933 bp[2] End 50,571,080 bp[2]
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right ventricle biceps brachii myocardium of left ventricle thoracic diaphragm vastus lateralis muscle Skeletal muscle tissue of biceps brachii body of tongue Skeletal muscle tissue of rectus abdominis muscle of leg gastrocnemius muscle Top expressed in digastric muscle sternocleidomastoid muscle temporal muscle triceps brachii muscle vastus lateralis muscle interventricular septum right ventricle myocardium of ventricle spermatocyte muscle of thigh More reference expression data BioGPS n/a
Gene ontology Molecular function transferase activity dihydrolipoyllysine-residue acetyltransferase activity protein binding pyruvate dehydrogenase (NAD+) activity acyltransferase activity identical protein binding dihydrolipoamide S-acyltransferase activity Cellular component mitochondrial pyruvate dehydrogenase complex myelin sheath pyruvate dehydrogenase complex mitochondrial matrix mitochondrion Biological process tricarboxylic acid cycle regulation of acetyl-CoA biosynthetic process from pyruvate sleep pyruvate metabolic process glucose metabolic process metabolism acetyl-CoA biosynthetic process from pyruvate cellular nitrogen compound metabolic process carbohydrate metabolic process Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 1737 235339 Ensembl ENSG00000150768 ENSMUSG00000000168 UniProt P10515 Q8BMF4 RefSeq (mRNA) NM_001931 NM_145614 RefSeq (protein) NP_001922 NP_001358960 NP_001358961 NP_001358962 NP_001358963 NP_001358964 NP_001358965 NP_001358966 NP_001358967 NP_001358968 NP_001358969 NP_001358970 NP_001358971 NP_663589 Location (UCSC) Chr 11: 112.02 – 112.06 Mb Chr 9: 50.55 – 50.57 Mb PubMed search [3] [4]
Wikidata
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Dihydrolipoyl transacetylase (or dihydrolipoamide acetyltransferase) is an enzyme component of the multienzyme pyruvate dehydrogenase complex. The pyruvate dehydrogenase complex is responsible for the pyruvate decarboxylation step that links glycolysis to the citric acid cycle. This involves the transformation of pyruvate from glycolysis into acetyl-CoA which is then used in the citric acid cycle to carry out cellular respiration.

There are three different enzyme components in the pyruvate dehydrogenase complex. Pyruvate dehydrogenase (EC 1.2.4.1) is responsible for the oxidation of pyruvate, dihydrolipoyl transacetylase (this enzyme; EC 2.3.1.12) transfers the acetyl group to coenzyme A (CoA), and dihydrolipoyl dehydrogenase (EC 1.8.1.4) regenerates the lipoamide. Because dihydrolipoyl transacetylase is the second of the three enzyme components participating in the reaction mechanism for conversion of pyruvate into acetyl CoA, it is sometimes referred to as E2.

In humans, dihydrolipoyl transacetylase enzymatic activity resides in the pyruvate dehydrogenase complex component E2 (PDCE2) that is encoded by the DLAT (dihydrolipoamide S-acetyltransferase) gene.^[5]