Discontinuous electrophoresis

DNA bands after electrophoresis

Discontinuous electrophoresis (colloquially disc electrophoresis[a]) is a type of polyacrylamide gel electrophoresis. It was developed by Ornstein and Davis.[2][1] This method produces high resolution and good band definition. It is widely used technique for separating proteins according to size and charge.[3]

  1. ^ a b Ornstein, Leonard (2006-12-16). "Disc Electrophoresis-I Background and Theory". Annals of the New York Academy of Sciences. 121 (2). Wiley: 321–349. doi:10.1111/j.1749-6632.1964.tb14207.x. ISSN 0077-8923. PMID 14240533. S2CID 28591995.
  2. ^ Williams, Donald E. & Reisfeld, Ralph A. (1964). "DISC Electrophoresis IN POLYACRYLAMIDE GELS". Annals of the New York Academy of Sciences. 121 (2): 372–381. Bibcode:1964NYASA.121..373W. doi:10.1111/j.1749-6632.1964.tb14210.x. PMID 14240536. S2CID 46152998.
  3. ^ PolyAcrylamide Gel Electrophoresis (PDF). davidson.edu.


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