The Eastleigh to Salisbury line is the railway line from Eastleigh (Hampshire) through Romsey to Salisbury (Wiltshire) in England. It was constructed by the London and South Western Railway in 1857 from Bishopstoke; the station's name was changed to Eastleigh in 1889. At Salisbury the line ran to Milford station on the south-eastern margin of the city, but in 1859 an extension to the present-day Salisbury station was built, and the lines from Andover through Salisbury to Yeovil were connected.
In 1865 the Andover and Redbridge Railway was opened, giving a direct connection from Southampton to Romsey, and in 1889 the missing link from Netley to Romsey was ready. In time this became the dominant route to Southampton, and the Eastleigh to Romsey section was greatly reduced in importance.
The route to Salisbury carried through trains from Bristol and South Wales, and became an important artery from South Wales for bunker coal for coal-fuelled shipping at Southampton and Portsmouth. Except for the Milford station, the entire route continues in use at the present.