| EcoRI | |||||||||
|---|---|---|---|---|---|---|---|---|---|
 | |||||||||
| Identifiers | |||||||||
| Symbol | EcoRI | ||||||||
| Pfam | PF02963 | ||||||||
| InterPro | IPR004221 | ||||||||
| SCOP2 | 1na6 / SCOPe / SUPFAM | ||||||||
| CDD | 79lll | ||||||||
| |||||||||
EcoRI (pronounced "eco R one") is a restriction endonuclease enzyme isolated from species E. coli. It is a restriction enzyme that cleaves DNA double helices into fragments at specific sites, and is also a part of the restriction modification system.[1] The Eco part of the enzyme's name originates from the species from which it was isolated - "E" denotes generic name which is "Escherichia" and "co" denotes species name, "coli" - while the R represents the particular strain, in this case RY13, and the I denotes that it was the first enzyme isolated from this strain.[citation needed]
In molecular biology it is used as a restriction enzyme. EcoRI creates 4 nucleotide sticky ends with 5' end overhangs of AATT. The nucleic acid recognition sequence where the enzyme cuts is G↓AATTC, which has a palindromic complementary sequence of CTTAA↓G.[2] Other restriction enzymes, depending on their cut sites, can also leave 3' overhangs or blunt ends with no overhangs.
- ^ Halford, S. E.; Johnson, N. P. (1980-11-01). "The EcoRI restriction endonuclease with bacteriophage lambda DNA. Equilibrium binding studies". The Biochemical Journal. 191 (2): 593–604. doi:10.1042/bj1910593. ISSN 0264-6021. PMC 1162251. PMID 6263250.
- ^ Nevinsky, Georgy A. (2021-01-29). "How Enzymes, Proteins, and Antibodies Recognize Extended DNAs; General Regularities". International Journal of Molecular Sciences. 22 (3): 1369. doi:10.3390/ijms22031369. ISSN 1422-0067. PMC 7866405. PMID 33573045.