Electroblotting

Schematic representation of a transfer stack.[1]

Electroblotting is a method in molecular biology/biochemistry/immunogenetics to transfer proteins or nucleic acids onto a membrane by using PVDF or nitrocellulose, after gel electrophoresis.[2][3] The protein or nucleic acid can then be further analyzed using probes such as specific antibodies, ligands like lectins, or stains. This method can be used with all polyacrylamide and agarose gels. An alternative technique for transferring proteins from a gel is capillary blotting.

  1. ^ Cite error: The named reference Dubey2014 was invoked but never defined (see the help page).
  2. ^ David Sheehan (30 April 2013). Physical Biochemistry: Principles and Applications. John Wiley & Sons. pp. 206–. ISBN 978-1-118-68748-2.
  3. ^ M. P. Bansal (1 January 2013). Molecular Biology and Biotechnology: basic experimental protocols. The Energy and Resources Institute (TERI). pp. 141–. ISBN 978-81-7993-379-4.