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Elisabeth of Culemborg (30 March 1475, the former slot of Hoogstraten - 9 December 1555, Culemborg), nicknamed 'Lady Elizabeth ('Vrouwe Elisabeth') was the last sovereign lord or lady of the fiefdom of Culemborg (promoted to a county by Charles V, Holy Roman Emperor shortly before her death), from 1504 until 1555. She was also the dame d'honneur of Archduchess Margaret of Austria.