In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism. The extraction process is extensively sterilized, and the culture can be typically used for two to three weeks.[1]
The major advantage of explant culture is the maintenance of near in vivo environment in the laboratory for a short duration of time. This experimental setup allows investigators to perform experiments and easily visualize the impact of tests.[2]
This ex vivo model requires a highly maintained environment in order to recreate original cellular conditions. The composition of extracellular matrix, for example, must be precisely similar to that of in vivo conditions in order to induce naturally observed behaviors of cells. The growth medium also must be considered, as different solutions may be needed for different experiments.[1][2]
The tissue must be placed and harvested in an aseptic environment such as sterile laminar flow tissue culture hood. The samples are often minced, and the pieces are placed in a cell culture dish containing growth media. Over time, progenitor cells migrate out of the tissue onto the surface of the dish. These primary cells can then be further expanded and transferred into fresh dishes through micropropagation.
Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding extracellular matrix to more accurately mimic the in vivo environment e.g. cartilage explant culture,[3] or blastocyst implant culture.[4]