| Site-specific recombinase Flp | |||||||
|---|---|---|---|---|---|---|---|
| Identifiers | |||||||
| Organism | |||||||
| Symbol | FLP1 | ||||||
| UniProt | P03870 | ||||||
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In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo. It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp) derived from the 2 μ plasmid of baker's yeast Saccharomyces cerevisiae.
The 34bp minimal FRT site sequence has the sequence
- 5'GAAGTTCCTATTCtctagaaaGAATAGGAACTTC3'
for which flippase (Flp) binds to both 13-bp 5'-GAAGTTCCTATTC-3' arms flanking the 8 bp spacer, i.e. the site-specific recombination (region of crossover) in reverse orientation. FRT-mediated cleavage occurs just ahead from the asymmetric 8bp core region (5'tctagaaa3') on the top strand and behind this sequence on the bottom strand.[1] Several variant FRT sites exist, but recombination can usually occur only between two identical FRTs but generally not among non-identical ("heterospecific") FRTs.[2][3]
- ^ Zhu XD, Sadowski PD (September 1995). "Cleavage-dependent ligation by the FLP recombinase. Characterization of a mutant FLP protein with an alteration in a catalytic amino acid". The Journal of Biological Chemistry. 270 (39): 23044–54. doi:10.1074/jbc.270.39.23044. PMID 7559444.
- ^ Schlake T, Bode J (November 1994). "Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci". Biochemistry. 33 (43): 12746–51. doi:10.1021/bi00209a003. PMID 7947678.
- ^ Turan S, Kuehle J, Schambach A, Baum C, Bode J (September 2010). "Multiplexing RMCE: versatile extensions of the Flp-recombinase-mediated cassette-exchange technology". Journal of Molecular Biology. 402 (1): 52–69. doi:10.1016/j.jmb.2010.07.015. PMID 20650281.