| GcvB RNA | |
|---|---|
 Predicted secondary structure and sequence conservation of GcvB | |
| Identifiers | |
| Symbol | GcvB |
| Rfam | RF00022 |
| Other data | |
| RNA type | Gene |
| Domain(s) | Bacteria |
| SO | SO:0000379 |
| PDB structures | PDBe |
The gcvB RNA gene encodes a small non-coding RNA involved in the regulation of a number of amino acid transport systems as well as amino acid biosynthetic genes. The GcvB gene is found in enteric bacteria such as Escherichia coli. GcvB regulates genes by acting as an antisense binding partner of the mRNAs for each regulated gene. This binding is dependent on binding to a protein called Hfq. Transcription of the GcvB RNA is activated by the adjacent GcvA gene and repressed by the GcvR gene.[1] A deletion of GcvB RNA from Y. pestis changed colony shape as well as reducing growth.[2] It has been shown by gene deletion that GcvB is a regulator of acid resistance in E. coli. GcvB enhances the ability of the bacterium to survive low pH by upregulating the levels of the alternate sigma factor RpoS.[3] A polymeric form of GcvB has recently been identified.[citation needed] Interaction of GcvB with small RNA SroC triggers the degradation of GcvB by RNase E, lifting the GcvB-mediated mRNA repression of its target genes.[4]
- ^ Urbanowski, ML; Stauffer LT; Stauffer GV (2000). "The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli". Mol Microbiol. 37 (4): 856–868. doi:10.1046/j.1365-2958.2000.02051.x. PMID 10972807. S2CID 811119.
- ^ McArthur SD, Pulvermacher SC, Stauffer GV (2006). "The Yersinia pestis gcvB gene encodes two small regulatory RNA molecules". BMC Microbiol. 6: 52. doi:10.1186/1471-2180-6-52. PMC 1557403. PMID 16768793.
- ^ Jin Y, Watt RM, Danchin A, Huang JD (2009). "Small noncoding RNA GcvB is a novel regulator of acid resistance in Escherichia coli". BMC Genomics. 10: 165. doi:10.1186/1471-2164-10-165. PMC 2676305. PMID 19379489.
- ^ Miyakoshi, Masatoshi; Chao, Yanjie; Vogel, Jörg (2015-06-03). "Cross talk between ABC transporter mRNAs via a target mRNA-derived sponge of the GcvB small RNA". The EMBO Journal. 34 (11): 1478–1492. doi:10.15252/embj.201490546. ISSN 1460-2075. PMC 4474525. PMID 25630703.