| FOLH1 | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| Identifiers | |||||||||||||||||||||||||||||||||||||||||||||||||||
| Aliases | FOLH1, FGCP, FOLH, GCP2, GCPII, NAALAD1, NAALAdase, PSM, PSMA, mGCP, folate hydrolase (prostate-specific membrane antigen) 1, folate hydrolase 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||
| External IDs | OMIM: 600934; MGI: 1858193; HomoloGene: 136782; GeneCards: FOLH1; OMA:FOLH1 - orthologs | ||||||||||||||||||||||||||||||||||||||||||||||||||
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| TAH molecule | |||||||||
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 Reaction Scheme of NAAG Degradation by GCPII: GCPII + NAAG → GCPII-NAAG complex → Glutamate + NAA | |||||||||
| Identifiers | |||||||||
| EC no. | 3.4.17.21 | ||||||||
| CAS no. | 111070-04-3 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
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TAH molecule, also known as N-acetyl-L-aspartyl-L-glutamate peptidase I (NAALADase I), NAAG peptidase, or prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene.[5] Human GCPII contains 750 amino acids and weighs approximately 84 kDa.[6]
GCPII is a zinc metalloenzyme that resides in membranes. Most of the enzyme resides in the extracellular space. GCPII is a class II membrane glycoprotein. It catalyzes the hydrolysis of N-acetylaspartylglutamate (NAAG) to glutamate and N-acetylaspartate (NAA) according to the reaction scheme to the right.[7][8]
Neuroscientists primarily use the term NAALADase in their studies, while those studying folate metabolism use folate hydrolase, and those studying prostate cancer or oncology, PSMA. All refer to the same protein glutamate carboxypeptidase II.
- ^ a b c GRCh38: Ensembl release 89: ENSG00000086205 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000001773 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ O'Keefe DS, Su SL, Bacich DJ, Horiguchi Y, Luo Y, Powell CT, et al. (November 1998). "Mapping, genomic organization and promoter analysis of the human prostate-specific membrane antigen gene". Biochimica et Biophysica Acta (BBA) - Gene Structure and Expression. 1443 (1–2): 113–127. doi:10.1016/s0167-4781(98)00200-0. PMID 9838072.
- ^ Cite error: The named reference
Barinka_2004was invoked but never defined (see the help page). - ^ Rojas C, Frazier ST, Flanary J, Slusher BS (November 2002). "Kinetics and inhibition of glutamate carboxypeptidase II using a microplate assay". Analytical Biochemistry. 310 (1): 50–54. doi:10.1016/S0003-2697(02)00286-5. PMID 12413472.
- ^ Mesters JR, Barinka C, Li W, Tsukamoto T, Majer P, Slusher BS, et al. (March 2006). "Structure of glutamate carboxypeptidase II, a drug target in neuronal damage and prostate cancer". The EMBO Journal. 25 (6): 1375–1384. doi:10.1038/sj.emboj.7600969. PMC 1422165. PMID 16467855.