The Goulburn Valley is a sub-region, part of the Hume region of the Australian state of Victoria. The sub-region consists of those areas in the catchment of the Goulburn River and other nearby streams, and is part of the Murray-Darling Basin. The Goulburn Valley is bordered on the south by the Great Dividing Range and to the north by the Murray River, the state border with New South Wales. The sub-region is one of Australia's most productive and intensively farmed areas and is predominantly irrigated.
Major regional centres of the Goulburn Valley are Shepparton, Seymour, Echuca, Benalla, Yarrawonga and Kyabram.