Halohydrin dehalogenase

A halohydrin dehalogenase is an enzyme involved in the bacterial degradation of vicinal halohydrins. In several species of bacteria, it catalyses the dehalogenation of halohydrins to produce the corresponding epoxides.[1] Different isoforms of the enzyme fall into one of three groups, A, B or C.[2] Halogenases of the same class are genetically similar, but differ greatly from halogenases from a different group.[2][3] Currently the most well-studied isoform is HheC which is purified from the bacterial species Agrobacterium radiobacter.[4] The ability to dehalogenate organic compounds as well as form enantiomeric selective epoxides have generated interest in the potential of this enzyme in the biochemical field.[5]

  1. ^ Fauzi AM, Hardman DJ, Bull AT (1996). "Biodehalogenation of low concentrations of 1,3-dichloropropanol by mono- and mixed cultures of bacteria". Appl Microbiol Biotechnol. 46 (5–6): 660–666. doi:10.1007/s002530050877. PMID 9008896. S2CID 43047193.
  2. ^ a b van Hylckama Vlieg JE, Tang LX, Lutje Spelberg JH, Smilda T, Poelarends GJ, Bosma T, van van Merode AE, Fraaije MW, Janssen DB (2001). "Halohydrin dehalogenases are structurally and mechanistically related to shortchain dehydrogenases/reductases". J Bacteriol. 183 (17): 5058–5066. doi:10.1128/jb.183.17.5058-5066.2001. PMC 95381. PMID 11489858.
  3. ^ You ZY, Liu ZQ, Zheng YG (2013). "Properties and biotechnological applications of halohydrin dehalogenases: current state and future perspectives". Appl Microbiol Biotechnol. 97 (1): 9–21. doi:10.1007/s00253-012-4523-0. PMID 23111599. S2CID 6884454.
  4. ^ "Biodegradation of halogenated pollutants". 7 July 2011.
  5. ^ Choi WJ, Choi CY (2005). "Production of chiral epoxides: epoxide hydrolase-catalyzed enantioselective hydrolysis". Biotechnol Bioprocess. 10 (3): 167–179. doi:10.1007/bf02932009. S2CID 84358740.