In biochemistry, immunostaining is any use of an antibody-based method to detect a specific protein in a sample. The term "immunostaining" was originally used to refer to the immunohistochemical staining of tissue sections, as first described by Albert Coons in 1941.[1] However, immunostaining now encompasses a broad range of techniques used in histology, cell biology, and molecular biology that use antibody-based staining methods.
- ^ Coons, Albert; Creech HJ; Jones, RN (1941). "Immunological properties of an antibody containing a fluorescent group". Proc Soc Exp Biol Med. 47 (2): 200–202. doi:10.3181/00379727-47-13084P. S2CID 101356912.