Impedance microbiology

Impedance microbiology is a microbiological technique used to measure the microbial number density (mainly bacteria but also yeasts) of a sample by monitoring the electrical parameters of the growth medium. The ability of microbial metabolism to change the electrical conductivity of the growth medium was discovered by Stewart[1] and further studied by other scientists such as Oker-Blom,[2] Parson[3] and Allison[4] in the first half of 20th century. However, it was only in the late 1970s that, thanks to computer-controlled systems used to monitor impedance, the technique showed its full potential, as discussed in the works of Fistenberg-Eden & Eden,[5] Ur & Brown[6] and Cady.[7]

  1. ^ Stewart, G.N. (1899). "The changes produced by the growth of bacteria in the molecular concentration and electrical conductivity of culture media". Journal of Experimental Medicine. 4 (2): 235–243. doi:10.1084/jem.4.2.235. PMC 2118043. PMID 19866908.
  2. ^ Oker-Blom, M (1912). "Die elektrische Leitfahigkeit im dienste der Bakteriologie". Zentralbl Bakteriol. 65: 382–389.
  3. ^ Parsons, L.B.; Sturges, W.S. (1926). "The possibility of the conductivity method as applied to studies of bacterial metabolism". Journal of Bacteriology. 11 (3): 177–188. doi:10.1128/JB.11.3.177-188.1926. PMC 374863. PMID 16559179.
  4. ^ Allison, J.B.; Anderson, J.A.; Cole, W.H. (1938). "The method of electrical conductivity in studies of bacterial metabolism". Journal of Bacteriology. 36 (6): 571–586. doi:10.1128/JB.36.6.571-586.1938. PMC 545411. PMID 16560176.
  5. ^ Fistenberg-Eden, R.; Eden, G. (1984). Impedance Microbiology. New York: John Wiley.
  6. ^ Ur, A.; Brown, D.F.J. (1975). Monitoring of bacterial activity by impedance measurements. New York: Chapter 5 in “New approaches to the identification of microorganisms”, edited by C. Heden & T. Illeni, John Wiley & Sons. pp. 63–71.
  7. ^ Cady, P. (1978). Progress in impedance measurements in microbiology. Springfield: Chapter 14 in “Mechanizing microbiology” edited by Anthony N. Sharpe & David S. Clark, Charles C. Thomas Publisher. pp. 199–239.