Jardine is a surname. Notable people with the surname include:
- James Jardine (engineer) (1776–1858), Scottish civil engineer, mathematician and geologist
- James Jardine (cricketer, born 1794) (1794–1872), English cricketer
- James Jardine (judge) (1846–1909), English cricketer, academic, barrister and judge
- James Jardine (Medal of Honor) (1837–1922), Union Army soldier and Medal of Honor recipient
- James Bruce Jardine (1870–1955), British soldier and diplomat
- James Willoughby Jardine (1879–1945), British judge and politician
- John E. Jardine (1838–1920) of David and John Jardine, US architect
- John Jardine (football coach) (died 1990), American football coach
- Sir John Jardine, 1st Baronet (1844–1919), Scottish Liberal politician and businessman
- Keith Jardine (born 1975), American mixed martial artist
- Lisa Jardine (1944–2015), British historian
- Nicholas Jardine (born 1943), British mathematician, philosopher of science and its history, and historian
- Ray Jardine, American author and adventurer
- Robert Jardine (disambiguation), several people:
- William Jardine (1784–1843), Scottish surgeon and businessman
- William Jardine (merchant), Scottish surgeon and merchant, of Jardine, Matheson & Co.
- Sir William Jardine, 7th Baronet (1800–1874), Scottish naturalist
- William Marion Jardine (1879–1959), U.S. Secretary of Agriculture, Ambassador to Egypt
- William Murray Jardine (born 1984), 24th Chief of Clan Jardine
- William Jardine, editor of the Farmer's Almanac