Jefferson School District may refer to:
- Jefferson County School District in Jefferson County, Alabama
- Jefferson Patriots School District in Phoenix, Arizona
- Jefferson County R-1 School District in Golden, Colorado
- Jefferson County School District in Monticello, Florida
- Jefferson City School District in Jefferson, Georgia
- Jefferson County School District in Louisville, Georgia
- Jefferson County School District 251 in Rigby, Idaho
- West Jefferson School District in Terreton, Indiana
- Jefferson–Scranton Community School District in Jefferson, Iowa
- Jefferson County School District in Louisville, Kentucky
- Jefferson County School District, in Fayette, Mississippi
- Jefferson County School District, in Jefferson New York
- Jefferson Area Local School District, in Jefferson, Ohio
- Jefferson Local School District, in West Jefferson, Ohio
- Jefferson Township Local School District, in Dayton, Ohio
- Jefferson School District, Oregon in Jefferson, Oregon
- Jefferson County School District (Oregon) in Madras, Oregon
- Jefferson County School District, in Dandridge, Tennessee
- Jefferson Independent School District in Jefferson, Texas
- Jefferson County School District in Charles Town, West Virginia
- Jefferson County School District in Jefferson, Wisconsin
- Jefferson School District in Tracy, California.
- Jefferson Schools in Monroe County, Michigan