John Jameson may refer to:
- John Jameson, businessman and whiskey distiller, founder of Jameson Irish Whiskey
- John Jameson (politician) (1802–1857), American lawyer and Congressman from Missouri
- John Jameson (colonel) (1751–1810), Continental Army soldier who helped discover Benedict Arnold's treason
- John Jameson (character), fictional Marvel character known as "the Man-Wolf"
- John Jameson (cricketer) (born 1941), English cricketer
- Johnny Jameson (born 1948), Northern Irish footballer
- John Gordon Jameson (1878–1955), British Member of Parliament for Edinburgh West, 1918–1922
- John Eustace Jameson (1853–1919), Member of Parliament for West Clare, 1895–1906
- John Paul Jameson (died 1700)