The accepted and known species of Nicotiana include:[1]
- Nicotiana acaulis Speg.[2]
- Nicotiana acuminata (Graham) Hook. – manyflower tobacco, many-flowered tobacco[2]
- Nicotiana africana Merxm.[2]
- Nicotiana alata Link & Otto – jasmine tobacco, sweet tobacco, winged tobacco, Persian tobacco, tanbaku (Persian)[2]
- Nicotiana ameghinoi Speg.[2]
- Nicotiana amplexicaulis N. T. Burb.[2]
- Nicotiana arentsii Goodsp.[2]
- Nicotiana attenuata Torrey ex S. Watson – coyote tobacco[2]
- Nicotiana azambujae L. B. Smith & Downs[2]
- Nicotiana benavidesii Goodsp.[2]
- Nicotiana benthamiana Domin[2] – benth, benthi
- Nicotiana bonariensis Lehm.[2]
- Nicotiana burbidgeae Symon[2]
- Nicotiana cavicola N. T. Burb.[2]
- Nicotiana clevelandii A. Gray[2] – Cleveland's tobacco
- Nicotiana cordifolia Phil.[2]
- Nicotiana corymbosa J. Rémy[2]
- Nicotiana cutleri D'Arcy[2]
- Nicotiana excelsior (J. M. Black) J. M. Black[2]
- Nicotiana forgetiana Hemsl.[2]
- Nicotiana forsteri Roem. & Schult. (previously designated N. debneyi)[2]
- Nicotiana fragrans Hooker[2]
- Nicotiana glauca Graham – tree tobacco, Brazilian tree tobacco, shrub tobacco, wild tobacco, tobacco plant, tobacco bush, tobacco tree, mustard tree[2]
- Nicotiana glutinosa L.[2]
- Nicotiana goodspeedii H.-M. Wheeler[2]
- Nicotiana gossei Domin[2]
- Nicotiana hesperis N. T. Burb.[2]
- Nicotiana heterantha Kenneally & Symon[2]
- Nicotiana ingulba J. M. Black[2]
- Nicotiana kawakamii Y. Ohashi[2]
- Nicotiana knightiana Goodsp.[2]
- Nicotiana langsdorffii Weinm.[2] – Langsdorff's tobacco
- Nicotiana linearis Phil.[2]
- Nicotiana longibracteata Phil.[2]
- Nicotiana longiflora Cav.[2] – longflower tobacco, long-flowered tobacco
- Nicotiana maritima H.-M. Wheeler[2]
- Nicotiana megalosiphon Van Huerck & Müll. Arg.[2]
- Nicotiana miersii J. Rémy[2]
- Nicotiana mutabilis Stehmann & Samir[2]
- Nicotiana nesophila I. M. Johnston[2]
- Nicotiana noctiflora Hook.[2]
- Nicotiana nudicaulis S. Watson[2]
- Nicotiana occidentalis H.-M. Wheeler[2] – native tobacco
- Nicotiana obtusifolia M. Martens & Galeotti (previously designated N. trigonophylla) – desert tobacco, punche, "tabaquillo"[2]
- Nicotiana otophora Griseb.[2]
- Nicotiana paa Mart. Crov.[2]
- Nicotiana palmeri A. Gray[2]
- Nicotiana paniculata L.[2] – smallflower tobacco, small-flowered tobacco
- Nicotiana pauciflora J. Rémy[2]
- Nicotiana petuniodes (Griseb.) Millán.[2]
- Nicotiana plumbaginifolia Viv.[2] – Tex-Mex tobacco
- Nicotiana quadrivalvis Pursh (replaces the following older classifications: N. multivalvis Lindl., N. plumbaginifolia Viv. var. bigelovii Torrey, N. bigelovii (Torrey) S. Watson)[2] – Indian tobacco
- Nicotiana raimondii J. F. Macbr.[2]
- Nicotiana repanda Willd. – fiddleleaf tobacco, fiddle-leafed tobacco[2]
- Nicotiana rotundifolia Lindl.[2]
- Nicotiana rustica L. – Aztec tobacco, strong tobacco, mapacho[2]
- Nicotiana setchellii Goodsp.[2]
- Nicotiana simulans N. T. Burb.[2]
- Nicotiana solanifolia Walp.[2]
- Nicotiana spegazzinii Millán[2]
- Nicotiana stenocarpa H.-M. Wheeler[2]
- Nicotiana stocktonii Brandegee[2]
- Nicotiana suaveolens Lehm. – Australian tobacco[2]
- Nicotiana sylvestris Speg. & Comes – woodland tobacco, flowering tobacco, South American tobacco[2]
- Nicotiana tabacum L. – common tobacco, domesticated tobacco, cultivated tobacco, commercial tobacco (grown for the production of cigars, cigarillos, cigarettes, chewing tobacco, dipping tobacco, snuff, snus, etc.)[2]
- Nicotiana thrysiflora Bitter ex Goodsp.[2]
- Nicotiana tomentosa Ruiz & Pav.[2]
- Nicotiana tomentosiformis Goodsp.[2]
- Nicotiana truncata D. E. Symon[2]
- Nicotiana umbratica N. T. Burb.[2]
- Nicotiana undulata Ruiz & Pav.[2]
- Nicotiana velutina H.-M. Wheeler[2]
- Nicotiana wigandioides Koch & Fintelm.[2]
- Nicotiana wuttkei Clarkson & Symon[2]
Additional species that are listed by APNI are: