Lowry protein assay

The Lowry protein assay is a biochemical assay for determining the total level of protein in a solution. The total protein concentration is exhibited by a color change of the sample solution in proportion to protein concentration, which can then be measured using colorimetric techniques. It is named for the biochemist Oliver H. Lowry who developed the reagent in the 1940s. His 1951 paper describing the technique is the most-highly cited paper ever in the scientific literature, cited over 300,000 times.[1][2][3]

  1. ^ Van Noorden, R.; Maher, B.; Nuzzo, R. (2014). "The top 100 papers". Nature. 514 (7524): 550–553. Bibcode:2014Natur.514..550V. doi:10.1038/514550a. PMID 25355343.
  2. ^ Kresge, N.; Simoni, R. D.; Hill, R. L. (2005). "The Most Highly Cited Paper in Publishing History: Protein Determination by Oliver H. Lowry". Journal of Biological Chemistry. 280 (28): e25.
  3. ^ Garfield, E. (1990). "The Most-Cited Papers of All Time, SCI 1945-1988. Part 1A. The SCI Top 100—Will the Lowry Method Ever Be Obliterated?" (PDF). Current Contents. 7: 3–14.