MRNA (guanine-N7-)-methyltransferase

RNMT
Available structures
PDB	Ortholog search: PDBe RCSB
List of PDB id codes
	3BGV, 3EPP, 5E9J, 5E9W, 5E8J
Identifiers
Aliases	RNMT, MET, RG7MT1, hCMT1c, CMT1, CMT1c, cm1p, hCMT1, hMet, MRNA-methyltransferase, RNA guanine-7 methyltransferase, N7-MTase
External IDs	OMIM: 603514; MGI: 1915147; HomoloGene: 2816; GeneCards: RNMT; OMA:RNMT - orthologs
Gene location (Human)
Chr.	Chromosome 18 (human)
End	13,764,556 bp
Gene location (Mouse)
Chr.	Chromosome 18 (mouse)
End	68,457,923 bp
RNA expression pattern
	Top expressed in
	testicle; ; amniotic fluid; ; lateral nuclear group of thalamus; ; middle temporal gyrus; ; Brodmann area 23; ; pars compacta; ; tendon; ; Achilles tendon; ; cartilage tissue; ; internal globus pallidus;
	Top expressed in
	secondary oocyte; ; morula; ; zygote; ; epiblast; ; primitive streak; ; embryo; ; substantia nigra; ; blastocyst; ; dentate gyrus of hippocampal formation granule cell; ; tail of embryo;
	More reference expression data
	More reference expression data
Gene ontology
Molecular function	methyltransferase activity; transferase activity; mRNA (guanine-N7-)-methyltransferase activity; protein binding; RNA binding;
Cellular component	mRNA cap binding complex; receptor complex; nucleus; nucleoplasm; fibrillar center; mRNA cap methyltransferase complex;
Biological process	mRNA processing; methylation; transcription by RNA polymerase II; RNA 5'-cap (guanine-N7)-methylation; cellular response to leukemia inhibitory factor; 7-methylguanosine mRNA capping;
	Sources:Amigo / QuickGO
Orthologs
	8731
	67897
	ENSG00000101654
	ENSMUSG00000009535
	O43148
	Q9D0L8
	NM_001308263; NM_003799; NM_001378132; NM_001378134; NM_001378135
	NM_001170953; NM_026440; NM_001361002; NM_001361003
	NP_001295192; NP_003790; NP_001365061; NP_001365063; NP_001365064
	NP_001164424; NP_080716; NP_001347931; NP_001347932
	Wikidata
View/Edit Human	View/Edit Mouse

Search
PMC	articles
PubMed	articles
NCBI	proteins

mRNA (guanine-N7-)-methyltransferase
mRNA (guanine-N7-)-methyltransferase
Identifiers
EC no.	2.1.1.56
CAS no.	56941-25-4
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / QuickGO
PMC
Search
PMC	articles
PubMed	articles
NCBI	proteins

In enzymology, a mRNA (guanine-N7-)-methyltransferase also known as mRNA cap guanine-N7 methyltransferase is an enzyme that catalyzes the chemical reaction

S-adenosyl-L-methionine + G(5')pppR-RNA

\rightleftharpoons

S-adenosyl-L-homocysteine + m₇G(5')pppR-RNA (mRNA containing an N⁷-methylguanine cap)

Thus, the two substrates of this enzyme are S-adenosyl methionine and G(5')pppR-RNA, whereas its two products are S-adenosylhomocysteine and m7G(5')pppR-RNA. This enzyme belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases.

In humans, mRNA cap guanine-N7 methyltransferase is encoded by the RNMT gene.^[5]^[6]^[7]

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000101654 – Ensembl, May 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000009535 – Ensembl, May 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Pillutla RC, Shimamoto A, Furuichi Y, Shatkin AJ (Jan 1999). "Human mRNA capping enzyme (RNGTT) and cap methyltransferase (RNMT) map to 6q16 and 18p11.22-p11.23, respectively". Genomics. 54 (2): 351–3. doi:10.1006/geno.1998.5604. PMID 9828141.
^ Pillutla RC, Yue Z, Maldonado E, Shatkin AJ (Sep 1998). "Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes". J Biol Chem. 273 (34): 21443–6. doi:10.1074/jbc.273.34.21443. PMID 9705270.
^ "Entrez Gene: RNMT RNA (guanine-7-) methyltransferase".

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000101654 – Ensembl, May 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000009535 – Ensembl, May 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid9828141-5] Pillutla RC, Shimamoto A, Furuichi Y, Shatkin AJ (Jan 1999). "Human mRNA capping enzyme (RNGTT) and cap methyltransferase (RNMT) map to 6q16 and 18p11.22-p11.23, respectively". Genomics. 54 (2): 351–3. doi:10.1006/geno.1998.5604. PMID 9828141.

[pmid9705270-6] Pillutla RC, Yue Z, Maldonado E, Shatkin AJ (Sep 1998). "Recombinant human mRNA cap methyltransferase binds capping enzyme/RNA polymerase IIo complexes". J Biol Chem. 273 (34): 21443–6. doi:10.1074/jbc.273.34.21443. PMID 9705270.

[entrez-7] "Entrez Gene: RNMT RNA (guanine-7-) methyltransferase".

[1]

[2]

[3]

[4]

[5]

[6]

[7]