Madurella mycetomatis is a main cause of eumycetoma,[1] an infection of human extremities and rarely the nervous system, in arid regions of east Africa and Asia. The origin is soil and its dark agar colonies are often sterile, although sclerotia are often produced, and short chains of 1-celled conidia sometimes occur.[2] A molecular assay distinguishes the four species based on rolling circle amplification of the internal transcribed spacer of the ribosomal DNA (ITS).[3]