| Matheniko Wildlife Reserve | |
|---|---|
IUCN category IV (habitat/species management area) | |
  | |
| Location | Moroto District Uganda |
| Nearest city | Mbale |
| Coordinates | 2°55′N 34°30′E / 2.917°N 34.500°E |
| Area | 1,520 square kilometres (590 sq mi) |
| Governing body | Uganda Wildlife Authority |
The Matheniko Wildlife Reserve is a conservation area in the Karamoja subregion of northeastern Uganda. It is the fifth most-threatened conservation protected area in Uganda.