Methylated-DNA--protein-cysteine methyltransferase (MGMT), also known as O6-alkylguanine DNA alkyltransferase AGT, is a protein that in humans is encoded by the MGMT gene.[5][6] MGMT is crucial for genome stability. It repairs the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine and prevents mismatch and errors during DNA replication and transcription. Accordingly, loss of MGMT increases the carcinogenic risk in mice after exposure to alkylating agents.[7] The two bacterial isozymes are Ada and Ogt.
- ^ a b c GRCh38: Ensembl release 89: ENSG00000170430 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000054612 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ Tano K, Shiota S, Collier J, Foote RS, Mitra S (January 1990). "Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine". Proc. Natl. Acad. Sci. U.S.A. 87 (2): 686–90. Bibcode:1990PNAS...87..686T. doi:10.1073/pnas.87.2.686. PMC 53330. PMID 2405387.
- ^ Natarajan AT, Vermeulen S, Darroudi F, Valentine MB, Brent TP, Mitra S, Tano K (January 1992). "Chromosomal localization of human O6-methylguanine-DNA methyltransferase (MGMT) gene by in situ hybridization". Mutagenesis. 7 (1): 83–5. doi:10.1093/mutage/7.1.83. PMID 1635460.
- ^ Shiraishi A, Sakumi K, Sekiguchi M (October 2000). "Increased susceptibility to chemotherapeutic alkylating agents of mice deficient in DNA repair methyltransferase". Carcinogenesis. 21 (10): 1879–83. doi:10.1093/carcin/21.10.1879. PMID 11023546.