N-terminal acetylation is the protein modification that occurs on the α-amino acid group at the N-termini of proteins. The backbone amino group on the first amino acid (α-amino group) on a protein N-terminus gets an acetyl group (-COCH3) via acetyl-CoA, and this process is catalyzed by enzymes called N-terminal acetyltransferases (NATs).[1] This changes the chemical properties by making the protein more hydrophobic. Adding an acetyl group on the N-terminus of proteins is to date not shown to be reversible.