NDH-2

Enzymatic reaction catalyzed by NDH-2. In yellow is represented the protein surface, sitting in the membrane (in gray)

NDH-2, also known as type II NADH:quinone oxidoreductase or alternative NADH dehydrogenase, is an enzyme (EC: 1.6.99.3) which catalyzes the electron transfer from NADH (electron donor) to a quinone (electron acceptor), being part of the electron transport chain.[1] NDH-2 are peripheral membrane protein, functioning as dimers in vivo, with approximately 45 KDa per subunit and a single FAD as their cofactor.[2]

NDH-2 are the only enzymes, with NADH dehydrogenase activity, expressed in the respiratory chain of some pathogenic organisms (e.g. Staphylococcus aureus), and for that they have been proposed as new targets for rational drug design.[3]

  1. ^ B.C. Marreiros, F. Calisto, P.J. Castro, A.M. Duarte, F. V. Sena, A.F. Silva, F.M. Sousa, M. Teixeira, P.N. Refojo, M.M. Pereira, Exploring membrane respiratory chains, Biochim. Biophys. Acta - Bioenerg. 1857 (2016) 1039–1067.
  2. ^ Kerscher, S., Dröse, S., Zickermann, V., and Brandt, U. (2007) The Three Families of Respiratory NADH Dehydrogenases, in Bioenergetics (Schäfer, G., and Penefsky, H. S., Eds.), pp 185-222, Springer Berlin Heidelberg.
  3. ^ F. V. Sena, A.P. Batista, T. Catarino, J.A. Brito, M. Archer, M. Viertler, T. Madl, E.J. Cabrita, M.M. Pereira, Type-II NADH: Quinone oxidoreductase from Staphylococcus aureus has two distinct binding sites and is rate limited by quinone reduction, Mol. Microbiol. 98 (2015) 272–288. doi:10.1111/mmi.13120.