Native chemical ligation

Native Chemical Ligation (NCL) is an important extension of the chemical ligation concept for constructing a larger polypeptide chain by the covalent condensation of two or more unprotected peptides segments.^[1] Native chemical ligation is the most effective method for synthesizing native or modified proteins of typical size (i.e., proteins< ~300 AA).^[2]

^ Dawson, P. E.; Muir, T. W.; Clark-Lewis, I.; Kent, S. B. (1994). "Synthesis of proteins by native chemical ligation". Science. 266 (5186): 776–778. Bibcode:1994Sci...266..776D. doi:10.1126/science.7973629. PMID 7973629.
^ Agouridas V, El Mahdi O, Diemer V, Cargoët M, Monbaliu JM, Melnyk O (June 2019). "Native Chemical Ligation and Extended Methods: Mechanisms, Catalysis, Scope, and Limitations". Chemical Reviews. 119 (12): 7328–7443. doi:10.1021/acs.chemrev.8b00712. PMID 31050890. S2CID 145023266.

[1] Dawson, P. E.; Muir, T. W.; Clark-Lewis, I.; Kent, S. B. (1994). "Synthesis of proteins by native chemical ligation". Science. 266 (5186): 776–778. Bibcode:1994Sci...266..776D. doi:10.1126/science.7973629. PMID 7973629.

[2] Agouridas V, El Mahdi O, Diemer V, Cargoët M, Monbaliu JM, Melnyk O (June 2019). "Native Chemical Ligation and Extended Methods: Mechanisms, Catalysis, Scope, and Limitations". Chemical Reviews. 119 (12): 7328–7443. doi:10.1021/acs.chemrev.8b00712. PMID 31050890. S2CID 145023266.

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