Nitro blue tetrazolium chloride

Nitro blue tetrazolium chloride
Names
IUPAC name
2,2'-bis(4-Nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride
Identifiers
3D model (JSmol)
ChEBI
ChEMBL
ChemSpider
ECHA InfoCard 100.005.517 Edit this at Wikidata
UNII
  • InChI=1S/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2 checkY
    Key: FSVCQIDHPKZJSO-UHFFFAOYSA-L checkY
  • InChI=1/C40H30N10O6.2ClH/c1-55-37-25-29(13-23-35(37)47-43-39(27-9-5-3-6-10-27)41-45(47)31-15-19-33(20-16-31)49(51)52)30-14-24-36(38(26-30)56-2)48-44-40(28-11-7-4-8-12-28)42-46(48)32-17-21-34(22-18-32)50(53)54;;/h3-26H,1-2H3;2*1H/q+2;;/p-2
    Key: FSVCQIDHPKZJSO-NUQVWONBAE
  • [Cl-].[Cl-].[O-][N+](=O)c1ccc(cc1)n2nc(n[n+]2c3ccc(cc3OC)c7ccc([n+]5nc(nn5c4ccc([N+]([O-])=O)cc4)c6ccccc6)c(OC)c7)c8ccccc8
Properties
C40H30Cl2N10O6
Molar mass 817.64 g/mol
Appearance yellow crystalline powder
Melting point 200 °C (392 °F; 473 K)
Hazards
Occupational safety and health (OHS/OSH):
Main hazards
 may be reactive based on presence of tetrazole group, nitro group and contiguous nitrogen atoms
Flash point not available
Lethal dose or concentration (LD, LC):
2 g/kg
Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa).
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Nitro blue tetrazolium is a chemical compound composed of two tetrazole moieties. It is used in immunology for sensitive detection of alkaline phosphatase (with BCIP). NBT serves as the oxidant and BCIP is the AP-substrate (and gives also dark blue dye).