Peptide microarray

Peptide microarray
Other namesPeptide chip, peptide array
UsesTo study binding properties, specificity and functionality and kinetics of protein-peptide or protein-protein interactions

A peptide microarray (also commonly known as peptide chip or peptide epitope microarray) is a collection of peptides displayed on a solid surface, usually a glass or plastic chip. Peptide chips are used by scientists in biology, medicine and pharmacology to study binding properties and functionality and kinetics of protein-protein interactions in general. In basic research, peptide microarrays are often used to profile an enzyme (like kinase, phosphatase, protease, acetyltransferase, histone deacetylase etc.), to map an antibody epitope or to find key residues for protein binding. Practical applications are seromarker discovery, profiling of changing humoral immune responses of individual patients during disease progression, monitoring of therapeutic interventions, patient stratification and development of diagnostic tools and vaccines.

An example of a peptide array used for epitope identification and specificity mapping.[1]
  1. ^ Hansen, Lajla Bruntse; Buus, Soren; Schafer-Nielsen, Claus (2013-07-23). "Identification and Mapping of Linear Antibody Epitopes in Human Serum Albumin Using High-Density Peptide Arrays". PLOS ONE. 8 (7): e68902. Bibcode:2013PLoSO...868902H. doi:10.1371/journal.pone.0068902. ISSN 1932-6203. PMC 3720873. PMID 23894373.