A Phred quality score is a measure of the quality of the identification of the nucleobases generated by automated DNA sequencing.[1][2] It was originally developed for the computer program Phred to help in the automation of DNA sequencing in the Human Genome Project. Phred quality scores are assigned to each nucleotide base call in automated sequencer traces.[1][2] The FASTQ format encodes phred scores as ASCII characters alongside the read sequences. Phred quality scores have become widely accepted to characterize the quality of DNA sequences, and can be used to compare the efficacy of different sequencing methods. Perhaps the most important use of Phred quality scores is the automatic determination of accurate, quality-based consensus sequences.
- ^ a b Ewing B; Hillier L; Wendl MC; Green P. (1998). "Base-calling of automated sequencer traces using phred. I. Accuracy assessment". Genome Research. 8 (3): 175–185. doi:10.1101/gr.8.3.175. PMID 9521921.
- ^ a b Ewing B, Green P (1998). "Base-calling of automated sequencer traces using phred. II. Error probabilities". Genome Research. 8 (3): 186–194. doi:10.1101/gr.8.3.186. PMID 9521922.